Anal Pap: Squamous Cell Carcinoma
The Anal Pap test is used as a screening tool in high-risk populations to identify patients who have
premalignant cytologic changes or malignancy in the anal epithelium.
Clinical History/Findings
The patient is a 41 year-old bisexual male with positive HIV status
The patient presented with a suspicious anal lesion and has a history of condylomas in the anal track.
Specimen Type
The specimen was collected using a moistened Dacron swab inserted 1.5 –2 inches into the anal canal,
sampling the anal-rectal squamocolumnar jucntion.The specimen is rinsed directly into 20 mLPreservCyt.
Cytologic Diagnosis
Cytologic Dx: Carcinoma In-Situ
Surgical F/U: Moderately Differentiated Squamous Cell Carcinoma
HPV Results: Positive for High Risk Oncogenic HPV Types
Submitted by:
Ann Marie West, BS, CT (ASCP)
Parkland Health & Hospital System
Dallas, Texas
The Anal Pap test is used as a screening tool in high-risk populations to identify patients who have
premalignant cytologic changes or malignancy in the anal epithelium.
Clinical History/Findings
The patient is a 41 year-old bisexual male with positive HIV status
The patient presented with a suspicious anal lesion and has a history of condylomas in the anal track.
Specimen Type
The specimen was collected using a moistened Dacron swab inserted 1.5 –2 inches into the anal canal,
sampling the anal-rectal squamocolumnar jucntion.The specimen is rinsed directly into 20 mLPreservCyt.
Cytologic Diagnosis
Cytologic Dx: Carcinoma In-Situ
Surgical F/U: Moderately Differentiated Squamous Cell Carcinoma
HPV Results: Positive for High Risk Oncogenic HPV Types
Submitted by:
Ann Marie West, BS, CT (ASCP)
Parkland Health & Hospital System
Dallas, Texas
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Slide 1: Abnormal
multinucleated squamous
cell. The enlarged nuclei
are hyperchromatic with
irregular nuclear borders,
macronucleoli, and
chromatin clumping.
multinucleated squamous
cell. The enlarged nuclei
are hyperchromatic with
irregular nuclear borders,
macronucleoli, and
chromatin clumping.
Slide 2: Small group of
pleomorphic cells with high
N/C ratios. The nuclei of the
cells are hyperchromatic,
have irregular contours, and
irregular macronucloli.
pleomorphic cells with high
N/C ratios. The nuclei of the
cells are hyperchromatic,
have irregular contours, and
irregular macronucloli.
Slide 3: Large sheet of
ceels with squamous
differentiation displaying
neculaer plemorhism,
irregular borders,
hypercromasia, chromatin
clumping, and irregular
macronucloli.
ceels with squamous
differentiation displaying
neculaer plemorhism,
irregular borders,
hypercromasia, chromatin
clumping, and irregular
macronucloli.
Slide 4: Anal Biopsy.
Moderately differentiated
squamous cell carcinoma
Moderately differentiated
squamous cell carcinoma
| The Texas Society of Cytology |
